Fig. 5

Increase of immune cells in the infarcted heart after HucMSCs treatment on day 7. A Representative immunofluorescence images of hearts stained with human mitochondrion and CD3/CD4 in MI-HucMSC groups. Green, HucMSCs; Red, CD3; Blue, DAPI. Scale bar = 50 µm. B Representative immunofluorescence images of hearts stained with CD3 and CD4 between MI-N.S and MI-HucMSC groups. Green, CD3; Red, CD4; Blue, DAPI. Scale bar = 75 µm. C Representative immunofluorescence images of hearts stained with CD4 and Ki67 between MI-N.S and MI-HucMSC groups. Red, CD4; Green, Ki67; Blue, DAPI. Yellow arrow indicates CD4⁺Ki67⁺ double positive cells. Scale bar = 75 µm. D Representative immunofluorescence images of hearts stained with CD4 and FoxP3 between MI-N.S and MI-HucMSC groups. Green, CD4; Red, FoxP3; Blue, DAPI. Yellow arrow indicates CD4⁺FoxP3⁺ double positive cells. Scale bar = 50 µm. E Statistical results of percent (%) of CD3⁺ or CD4⁺ T cells between MI-N.S and MI-HucMSC groups. F Statistical results of cell counts of CD4⁺Ki67⁺ T cells between MI-N.S and MI-HucMSC groups. G Statistical results of cell counts of CD4⁺FoxP3⁺ Tregs between MI-N.S and MI-HucMSC groups. H The flow diagram of the heart digestion and strategy of flow cytometry analysis. I Statistical results of cell counts of immune cells between MI-N.S and MI-HucMSC groups. *p < 0.05 and **p < 0.01 versus MI-N.S group