Fig. 2
The kidney pathologic injuries of rats with passive Heymann nephritis and those after hESC-IMRC treatments. The kidneys of rats with passive Heymann nephritis showed diffusive granular staining of IgG (a) and C3 (b) along the glomerular capillary walls (× 400), glomerular basement membrane thickening and scattered spike formation (c), and decreased level of nephrin (d) (A, × 400). After hESC-IMRC treatments, the deposits of IgG (e, i) and C3 (f, j), the thickening and spikes (g, k) of basement membrane were alleviated, and the levels of nephrin were increased (h, l), in both the high-dose IMRC group (6 × 106 cells) and the low-dose IMRC group (3 × 106 cells). No obvious kidney injury was observed in negative controls (m–p). Nephrin expression in kidneys was reduced in rats of Heymann nephritis (B), which was recovered in both the low-dose (3 × 106 cells, T1, n = 8) and high-dose (6 × 106 cells, T2, n = 8) groups of IMRC treatments. *P < 0.05; **P < 0.01; ***P < 0.001