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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Activin B-activated Cdc42 signaling plays a key role in regulating adipose-derived mesenchymal stem cells-mediated skin wound healing

Fig. 8

Activin B-induced ADSCs proliferation by activating Cdc42-Erk-Srf pathway. A Genes expression related to MAPK signaling in Act B+ADSCs (Cdc42N17) group. B qPCR analysis was performed to confirm the relative mRNA expression level of Srf in four groups. C, E Western blot assays were performed to detect the level of Srf and phosphorylation Erk (p-Erk) in ADSCs, activin B+ADSCs, ADSCs (SCH772984) (Erk1/2 inhibitor) or ADSCs (Cdc42N17) treated with or without activin B, respectively. D, F The band and the relative quantification levels of the expression of Srf and phosphorylation Erk were analyzed. G Representative fluorescence imaging of EdU staining of ADSCs and ADSCs treated with Srf inhibitor CCG-100602 or Erk1/2 inhibitor SCH772984 and with/without activin B at 6 h. Scale bar = 50 μm. H The proliferation rates were quantified by calculating the percentage of EdU-positive cells. All values are expressed as mean ± SD from three independent repeats. *P < 0.05

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