Fig. 4

In vitro functional study of hUC-MSCs sheet. A Cytokine expression levels of hUC-MSCs sheet; B Flow cytometric analyses showed that lymphocytes (labeled by BRDU) had less proliferation rate when co-culturing with hUC-MSCs from hUC-MSCs sheet; C lymphocytes co-culturing with hUC-MSCs from hUC-MSCs sheet also had smaller Th1 positive population; D Phase contrast images of HUVEC cultured with hUC-MSCs conditional medium and negative control with the superposition of vectorial objects obtained from computer analysis using the customized “Angiogenesis Analyser” for ImageJ; E “Number of Junctions” and “Total Length” results calculated by ImageJ. ***p < 0.001