Fig. 2

Intravenously transplanted MSCs attenuated endoplasmic reticulum stress-induced apoptosis. A and B. Representative TEM images and long-axis length of dilated endoplasmic reticulum (ER) of ventricular tissue in mice administered with PBS (control), Dox/PBS, Dox/Single dose, or Dox/Consecutive doses on day 14. The dilated ER was identified by a yellow arrow. Scale bar, 1 μm, (n = 5). ^****P < 0.0001 vs Control, ^####P < 0.0001 vs Dox/PBS, ^&&&&P < 0.0001 vs Dox/Single dose. C. Representative immunofluorescence images of cardiac troponin I (cTnI, red) and GRP78 antibody (green) binding to ventricular tissue of mice administered with PBS (control), Dox/PBS, Dox/Single dose, or Dox/Consecutive doses on day 14. Scale bar, 100 μm. D. Statistics of the fluorescence intensity of GRP78 in each group on day 14 (n = 5). ^***P < 0.001, ^****P < 0.0001 vs Control, ^&&P < 0.01 vs Dox/Single dose. E and F. Western Blotting and quantified protein expression of the ER stress-induced apoptosis pathway in ventricular tissue of mice administrated with PBS (Control), Dox/PBS, Dox/Single dose on days 7 and 14, (n = 5). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 vs Control, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 vs Dox/PBS, ^&&&P < 0.001, ^&&&&P < 0.0001 vs Dox/Single dose. G and H. TUNEL assay (green) and apoptotic proportion of ventricular tissue in mice administrated with PBS (Control), Dox/PBS, Dox/Single dose, or Dox/Consecutive doses on day 14. Scale bar, 50 μm (n = 5). ^****P < 0.0001 vs Control, ^####P < 0.0001 vs Dox/PBS, ^&&&&P < 0.0001 vs Dox/Single dose. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test, unless specifically indicated