Fig. 7
From: Intravenously transplanted mesenchymal stromal cells: a new endocrine reservoir for cardioprotection
MiR-181a-5p Agomir improves the cardioprotective ability of MSCs. A. Experimental design of an in vitro study with miR181a-5p Agomir. B. Representative immunofluorescence images of antibody to α-Actinin (Red) and GRP78 (green) binding to cardiomyocytes exposed to Dox/MSC-EVs-AgomirNC, or Dox/ MSC-EVs-AgomirmiR−181a−5p. Scale bar, 50 μm. C. Statistics of GRP78 expression of cardiomyocytes exposed to Dox/MSC-EVs-AgomirNC, or Dox/MSC-EVs-AgomirmiR−181a−5p (n = 3). ***P < 0.001 vs Dox/MSC-EVs-AgomirNC. Data were analyzed by unpaired two-tailed Student’s t-test. D and E. Western blotting and quantitative protein expression of ER stress-induced apoptosis in cardiomyocytes exposed to PBS (Control), Dox/PBS, Dox/MSC-EVs-AgomirNC, or Dox/MSC-EVs-AgomirmiR−181a−5p, (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs Control, #P < 0.05, ##P < 0.01, ####P < 0.0001 vs Dox/PBS, &&P < 0.01, &&&P < 0.001, &&&&P < 0.0001 vs Dox/MSC-EVs-AgomirNC. Data were analyzed by the one-way analysis of variance followed by Bonferroni post hoc test. F. Representative bright-field images of cardiomyocytes exposed to Dox/MSC-EVs-AgomirNC, or Dox/MSC-EVs-AgomirmiR−181a−5p. Scale bar, 100 μm. G. Representative images and quantitative proportion of TUNEL-positive (green) cardiomyocytes exposed to PBS (Control), Dox/PBS, Dox/MSC-EVs-AgomirNC, or Dox/MSC-EVs-AgomirmiR−181a−5p. Scale bar, 50 μm (n = 5). **P < 0.01, ****P < 0.0001 vs Control, #P < 0.05, ####P < 0.0001 vs Dox/PBS, &&P < 0.01 vs Dox/MSC-EVs-AgomirNC. Data were analyzed by one-way ANOVA followed by Bonferroni post hoc test, unless specifically indicated