Fig. 2

Effects of ASCs on protein levels of αSMA and ECM remodeling enzymes, investigated by western blot at day 10. The quantified data are based on lysates from three independent experiments. The same three ASC donors were included in experiment 1 and 2. In experiment 3, two additional ASCs donors were used (n = 5). A–D Representative images of blots are shown. For instance, the images found in A represent a single blot (Blot A), which has been cut into three parts prior to incubation with anti-αSMA, anti-TubB, and anti-Vinc, respectively. The same is the case for the blots shown in B–D. Each replicate lane in the shown blots for NHDF Paracrine + TGF-β, Juxtacrine + TGF-β, and ASC + TGF-β represent lysates from co-cultures with three different ASC donors, respectively. The quantified bands of target proteins were related to the mean of the loading controls. Protein lysates from each experiment were loaded onto four gels/blots (designated A-D) as illustrated. Significance level: *p < 0.05, compared to NHDF + TGF-β. Abbreviations: TubB, Beta tubulin; Vinc, Vinculin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ActB, beta actin