Fig. 3

Effects of ASCs on ECM formation, measured in supernatants by ELISA at day 3, 6, 10, and 13. A, left) ECM formation by TGF-β stimulated NHDF monocultures, compared to unstimulated NHDF monocultures. Based on data from two experiments, with a minimum of four replicate wells per experiment per condition. Significance level: *p < 0.05. A, right) Quantification of cell numbers in TGF-β stimulated and unstimulated NHDF monocultures, based on DAPI staining. Eight replicate wells were stained per condition per day. Data for day 3, 6, and 13 are based on one experiment. Data for day 10 are based on two experiments. Significance level: *p < 0.05. B) ECM formation in co-cultures, compared to the sum of the levels in NHDF monocultures and ASC monocultures. Based on data from two experiments with a minimum of four replicate wells per experiment per condition, three ASC donors were included in experiment 1 and measurements were performed at day 3, 6, 10, and 13. In experiment 2, two ASC donors were included, and measurements were performed at day 6 and 10 (n = 5 for day 6 and 10, n = 3 for day 3 and 13). Significance level: juxtacrine co-cultures vs. the sum of monocultures: *p < 0.05. Paracrine co-cultures vs. the sum of monocultures: ^#p < 0.05