Fig. 1
From: High-efficient serum-free differentiation of endothelial cells from human iPS cells
Endothelial cell differentiation in monolayer culture. A Schematic illustration of the differentiation protocol. B Bright-field images of different time points (day -2 to day 6) of hiPSC-ECs differentiation. Scale bar = 100 μm. C Representative flow cytometric scatter plots of negative control and hiPSC-ECs stained for CD31-APC:VE-Cadherin-PE endothelial markers. Ticker marks indicate bi-exponential presentation of the data. D Bar diagrams representing biological replicates (n = 6) of differentiated hiPSC-ECs on day 6, analyzed by flow cytometry for CD31-APC:VE-Cadherin-PE. E Numbers of hiPSC-ECs in million / cm.2 on day 6 (n = 6). F Immunostaining of hiPSC-ECs on day 6 of hiPSC-ECs differentiation for CD31-APC (yellow), VE-Cadherin-AlexaFluor488 (green), von Willebrand factor (red) and Hoechst dye (blue). Scale bar = 100 μm