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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: High-efficient serum-free differentiation of endothelial cells from human iPS cells

Fig. 5

Differentiation of hiPSCs to endothelial cells in bioreactor suspension culture using the protocol optimized for 2D. A Bright-field images of cell aggregates formed in a suspension culture of NP0040 hiPSCs from day -4 to day 6 at 10X magnification. Scale bars: 100 μm. B Left panel scatter plot shows the fraction of CD31-APC:VE-Cardherin-PE positive cells at day 6 (red), analyzed by flow cytometry in 3 technical replications. Results of unstained controls are shown in gray. C Bar chart represents the fraction of positive cells as mean ± S.D. from 3 technical replications (isotype control shown in gray and staining shown in red). D Expression of CD31-APC (yellow), VE-Cadherin-AF488 (green) and von Willebrand factor (vWF; red), and Hoechst 33,342 against nuclei (blue) in hiPSC-ECs clusters at day 6 of differentiation. Images were obtained with a SP8 Leica confocal microscope. hiPSC-ECs clusters were stained as whole mounts. Scale bars: 100 μm

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