Fig. 4

Differentiation of hPSC-EB-CD144⁺ into hPSC-BCs. A Phase-contrast microscopy images of three hPSC-derived blast colonies (hPSC-BCs) A29, SA01 and H1 cell lines. Scale bar 100 µm. B, C Flow cytometry analysis of day 6 hPSC-BCs for hematoendothelial (CD309, CD143 and CD34), endothelial (CD144 and CD31) and hematopoietic markers (CD43, CD45 and CD41) as simple (B) and double labelling (C), n = 15 for every hPSC line and marker. D–H Expression of hematopoietic and endothelial transcription factors in CD144⁺-EBs, hPSC-ECs and hPSC-BCs: SCL (D), GATA2 (E), RUNX1c (F), GATA1 (G) and HOXA3 (H). Data are represented as mean ± SEM, n = 5 independent experiment for each cell line. *P value < 0.05; **P value < 0.005 and ***P value < 0.0005