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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Endothelial and hematopoietic hPSCs differentiation via a hematoendothelial progenitor

Fig. 5

Functional characterization of hematopoiesis. A Phase-contrast microscopy images of myeloid, CFU-GEMM (top image) and CFU-GM (middle image) (image above right), and erythroid colonies, BFU-E (bottom image), derived from hPSC-BCs. Scale bar: 100 µm. B Number of hematopoietic colonies per 105 hPSC-BCs seeded cells in methylcellulose (A29 n = 7, SA01 n = 6 and H1 n = 10). C Percentage of every type of myeloid and erythroid colony per hPSC line. D Ratio of gamma/epsilon and E epsilon/beta hemoglobin expression of the BFU-E and CFU-GEMM derived from hPSCs. F Presence of human DNA in the mouse BM was assessed by qPCR with primers amplifying specifically the human gene CD45. Data are represented as mean ± SEM. For the in vivo experiment: ND: non-detected, n = 2 for CD34+ and PBS and n = 5 for CD144+-EBs. For all the other histograms: n = 5 independent experiments for each cell line. *P value < 0.05; **P value < 0.005 and ***P value < 0.0005

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