Fig. 3

Fbxw11 reduces the in vivo repopulating capacity of HSPCs at steady state. A Experimental design of competitive reconstitution experiments. B The PB engraftment of GFP⁺ donor cells in recipient mice was detected by FACS one month after transplantation. The typical FACS results are shown (on the left), and the reconstitution efficiency is plotted (on the right), n = 5. C The PB engraftment of GFP⁺ donor cells in recipient mice was monitored monthly by FACS for 5 months, n = 5. D Mice were killed five months after first-round transplantation, and the engraftment of GFP⁺ cells in PB, BM and spleen cells was analyzed by FACS, n = 5. E GFP⁺ cells were sorted from the BM of first-round recipient mice five months after transplantation and transplanted into second-round recipient mice with competitor cells. The PB engraftment of GFP⁺ cells was monitored monthly for 4 months by FACS, n = 5. The data are representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001