Fig. 4

Lineage differentiation of HSPCs by Fbxw11 overexpression. A, B The lineages of donor-derived PB GFP⁺ cells were analyzed by FACS one month after first-round transplantation. The percentages of B220⁺ B cells, CD3⁺ T cells (A) and Gr-1⁺CD11b⁺ granulocytes (B) are shown. Typical FACS results (upper) and the percentages (lower) are shown. C–E The lineages of donor-derived PB GFP⁺ cells were analyzed five months after first-round transplantation. The percentages of B220⁺ B cells (C), PB CD3⁺ T cells (D) and Gr-1⁺CD11b⁺ granulocytes (E) are shown. F LSK cells were infected with control or recombinant retrovirus. LSK cells were sorted after 48 h, and the expression of myeloid-associated genes was studied by quantitative real-time PCR. *p < 0.05, **p < 0.01, ***p < 0.001