Fig. 5
From: Fbxw11 impairs the repopulation capacity of hematopoietic stem/progenitor cells
The effects of Fbxw11 on heterogeneous HSPC subpopulations at single-cell resolution. A Gating strategy for cell sorting. HSPCs were defined as live, GFP+Lin–c-Kit+Sca-1+ (LSK) cells. B In total, 12,229 successfully profiled LSK single cells (5297 LSK-Con and 6932 LSK-Fbxw11d) underwent t-SNE analysis. C Pseudotime analysis applied to the t-SNE/Seurat clusters defining their developmental relationship. D Heatmap shows scaled expression (log TPM values) of cluster-specific gene sets of each cluster in all clusters. AUC cutoff ≥ 0.85. For cluster 0 to cluster 3, only the top 10 cluster-specific genes are shown. E The distribution of each cluster in LSK-Con and LSK-Fbxw11d is shown