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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Mesenchymal stromal cells pretreated with proinflammatory cytokines enhance skin wound healing via IL-6-dependent M2 polarization

Fig. 1

The S-IT MSCs promotes more efficient polarization of macrophages toward the M2 phenotype than the S-MSCs. Bone marrow-derived macrophages were cultured in M-CSF for 7 days followed by culture with DMEM, the S-MSCs and the S-IT MSCs for 24 h. Cultured cells were collected and immunostained with CD206-APC and CD86-PE followed by flow cytometry analysis. The concentrations of several cytokines in the S-MSCs and S-IT MSCs were determined by ELISA. AC Percentage of CD206-positive and CD86-positive cells in macrophages after treatment with DMEM, S-MSCs, and S-IT MSCs by flow cytometry. D IL-6 secretion by MSCs after stimulation by various inflammatory factors. E IL-6 expression after IL-6 was silenced in IT MSCs using siRNA. F, G Expression of IL-4 and IL-13 in the S-IT MSCs. The results represent three independent experiments. *P < 0.05; **P < 0.001, and ***P < 0.0001

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