Fig. 2

Silencing IL-6 inhibits IT MSCs-mediated induction of M2 macrophages. Macrophages were cultured with the S-siNC-MSCs, S-siNC-IT MSCs, S-siIL-6-IT MSCs (the supernatant of IT MSCs after IL-6 knockdown), and S-siIL-6-IT MSCs-recIL-6 (the supernatant derived from siIL-6-IT MSCs after adding 100 ng/mL recombinant IL-6). A, B The percentage of CD206-positive and CD86-positive cells in macrophages after treatment with the above supernatant groups according to flow cytometry. C The secretion of IL-10, IL-4, IL-13, and TNF-α in the supernatants of each group of MSCs-treated macrophages was determined using ELISA. Data are representative of three independent experiments. *P < 0.05; **P < 0.001, and ***P < 0.0001