Fig. 4

IL-6 enhances the phagocytic and angiogenic ability of M2 macrophages. A, B Immunofluorescence staining of M2 macrophages endocytosed with FITC-labeled dextran (green). C Quantitative analysis of the number of nodes using ImageJ software in the angiogenesis assay. D Representative images of the tube network in the angiogenesis assay. Images were acquired at 12 h. E The concentration of VEGF derived from macrophages pretreated with the supernatants of each group of stem cells was detected by ELISA. F VEGF gene expression of wound tissue on day 3 in vivo, n = 4 mice for each group. Data are representative of three independent experiments. *P < 0.05; **P < 0.001, and ***P < 0.0001. Scale bar 100 = μm