Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: A long-term anti-inflammation markedly alleviated high-fat diet-induced obesity by repeated administrations of overexpressing IL10 human umbilical cord-derived mesenchymal stromal cells

Fig. 6

IL10-MSCs treatments repressed obesity-induced lipid accumulation and inflammation in white adipose tissue. A The H&E of epididymal and subcutaneous white adipose tissues. B-C The sizes of epididymal and subcutaneous adipocytes were analyzed by Image J software. D The mRNA expressions of genes regarding adipose synthesis were analyzed by RT-PCR. E–F The immunostaining of infiltrating macrophages (F4/80 positive cells) showed HFD feeding significantly increased the aggregations of F4/80 positive macrophages in adipose tissue, which was alleviated by MSCs and IL10-MSCs treatment, especially by latter. G The transcription levels of TNFα, IL1β, IL6, IL4, and IL10 in white adipose tissue were detected by RT-PCR. HFD feeding significantly promoted the transcription levels of TNFα, IL1β, and IL6, while decreased the transcription levels of IL4 and IL10. MSCs and IL10-MSCs treatments could inhibit the expressions of HFD-induced pro-inflammatory factors and increased the anti-inflammatory factors. (N = 6 per group). (* p < 0.05; ** p < 0.01; ***p < 0.001)

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512