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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Modeling of mitochondrial bioenergetics and autophagy impairment in MELAS-mutant iPSC-derived retinal pigment epithelial cells

Fig. 5

Upregulation of Prom1 expression and inhibition of AMPKα signaling in MELAS iPSC-derived RPE cells. A Differentiated control iPSC-RPE, iPSC-RPE hetlow and iPSC-RPE hethigh cells were treated with 20 μm CCCP for 6 h, 20 μm FG4592 overnight, and 10 μm Erastin for 6 h. Cell lysates were analyzed by Western Blotting using indicated antibodies, and cropped representative blots are presented. B Densitometric analysis of LC3-II/LC3-I ratio. C phospho-AMPKα/total-AMPKα ratio. D Densitometric analysis of Prom1/actin ratio. Full-length gels are presented in Additional file 1

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