Fig. 6
Impaired autophagy flux and defective degradation of LC3, p62, and PINK1 in MELAS iPSC-derived-RPE cells. A Cropped representative Western Blotting of LC3-I and LC3-II for the study of autophagy flux in control IPSC-RPE, iPSC-RPE-hetlow, and iPSC-RPE-hethigh treated with or without CCCP, 20 μm for 3 h in the presence or absence of bafilomycin, 100 nM for 3 h. B Schematic for quantifying autophagy flux. C Quantification of LC3 degradation. D LC3 synthesis. (E) LC3-II/LC3-I ratio. F PINK1 degradation. G PINK1 synthesis. H Cropped representative Western Blotting of p62 and actin under identical experimental conditions. I Quantification of p62 degradation. J p62 synthesis. K p62 accumulation. Full-length gels are presented in Additional file 1