Fig. 7
Activation of STAT3 correlates with inhibition of lysosomal cathepsin D and increased expression of PGC-1α in MELAS-RPE cells. A control iPSC-RPE, iPSC-RPE-hetlow, and iPSC-RPE-hethigh cells were treated with 20 μm CCCP in the presence and absence of bafilomycin. Cell lysates were analyzed by Western Blotting using specified antibodies and cropped representative blots are presented. B Quantification of active/pro-cathepsin D ratio. C Quantification of cathepsin D degradation. D Quantification of PGC-1α/actin ratio. E Western blotting with pY705 STAT3 and total STAT3 antibodies. E Quantification of pY705 STAT3/total-STAT3 ratio. Full-length gels are presented in Additional file 1