Fig. 9

Time courses of circulatory, inflammatory and apoptotic biomarkers. A Analytical result of flow cytometric analysis of circulatory level of Ly6G+ cells by day 28 after ICH induction, *versus other groups with different symbols (†, ‡, §), p < 0.0001. B Analytical result of flow cytometric analysis of circulatory level of CD11b/c+ cells by day 28 after ICH induction, *versus other groups with different symbols (†, ‡, §), p < 0.0001. C Analytical result of flow cytometric analysis of circulatory level of myeloperoxidase (MPO) + cells by day 28 after ICH induction, *versus other groups with different symbols (†, ‡, §), p < 0.0001. D Analytical result of flow cytometric analysis of circulatory level of early (AN-V+ /PI− cells) apoptosis of mononuclear cells, *versus other groups with different symbols (†, ‡, §), p < 0.0001. E Analytical result of flow cytometric analysis of circulatory level of late (i.e., AN-V+ /PI+ cells) apoptosis of mononuclear cells, *versus other groups with different symbols (†, ‡, §), p < 0.0001. F ELISA showed the circulating level of tumor necrosis factor (TNF)-α by day 2, *versus other groups with different symbols (†, ‡, §), p < 0.0001. G ELISA showed the circulating level of interleukin (IL)-6 by day 2, *versus other groups with different symbols (†, ‡, §), p < 0.0001. H ELISA showed the circulating level of TNF-α by day 28, *versus other groups with different symbols (†, ‡, §), p < 0.0001. I ELISA showed the circulating level of IL-6 by day 28, *versus other groups with different symbols (†, ‡, §), p < 0.0001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 6 for each group). Symbols [(*, †, ‡, §) indicate significance (at 0.05 level). IA = intra-carotid arterial administration; IV intravenous (i.e., tail vein) administration; SC = sham control; ICH = intracranial hemorrhage; Ir-MNa-hUC-MSC = ironic magnetic nanoparticles/human umbilical cord-derived mesenchymal stem cells