Fig. 5

Inhibition of OGA induces erythroid differentiation in K562 cells. A Percentages of CD71⁺ and CD235a⁺ cells in OGAi, OGTi, and control (pLenti) cells at various times of culture in the optimized EPO-based medium. B Representative morphology of differentiated cells on Wright-stained cytospin slides before imatinib pre-exposure (day −1) and after 10 days of culture in EPO-based medium (upper) and its scoring for percentages of cells in different stages from at least 300 cells (lower). Scale bar = 30 μm. C Western blot analysis of cellular O-GlcNAc, OGA, and OGT levels upon terminal erythroid differentiation at various times (day −1 to day 10). Blots were reprobed with anti-β-actin antibody to establish a loading control. D Quantitative analysis of O-GlcNAc, OGA, and OGT levels by densitometry after normalization to the loading control is shown. Data are mean ± SD (n = 3). ^*P < 0.05 versus pLenti cells on the same day of culture; two-sided Student's t test