Fig. 6

Inhibition of OGA and OGT affects erythroid-associated proteins and globin production in K562 cells. A Western blot analysis of erythroid-associated proteins, including BCL11A, KLF1, GAT1, and GATA2, and globin proteins, including α-, β-, and Ɣ-globin, in OGAi, OGTi, and control (pLenti) cells upon erythroid differentiation in EPO-based medium at various times (day −1 to day 10). Blots were reprobed with anti-β-actin antibody to establish a loading control. B Quantitative analysis of erythroid-associated and globin proteins by densitometry after normalization to the loading control is shown. Data are mean ± SD (n = 3). ^*P < 0.05 versus pLenti cells on the same day of culture; two-sided Student's t test. C qPCR of genes encoding key proteins in A. Data were normalized to GAPDH and represented in a heatmap