Fig. 8

Effects of BCL11A inhibition on the O-GlcNAc-mediated erythroid-associated and globin proteins in K562 cells. A Western blot analysis of erythroid-associated proteins, including BCL11A, KLF1, GAT1, and GATA2, and globin proteins, including α-, β-, and Ɣ-globin, in OGAi, BCL11Ai/OGAi, and control (pLenti) cells upon erythroid differentiation in EPO-based medium at various times (day −1 to day 10). Blots were reprobed with anti-β-actin antibody to establish a loading control. The dashed lines separate juxtapose lanes taken from the same experiment and the same blot image. B Quantitative analysis of erythroid-associated and globin proteins by densitometry after normalization to the loading control is shown. Data are mean ± SD (n = 3 or 4). ^*P < 0.05 versus pLenti cells on the same day of culture; one-way ANOVA with Tukey’s posttest. ^#P < 0.05 versus OGAi cells on the same day of culture; one-way ANOVA with Tukey’s posttest. C qPCR of genes encoding key proteins in A. Data were normalized to GAPDH and represented in a heatmap