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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: SNTA1-deficient human cardiomyocytes demonstrate hypertrophic phenotype and calcium handling disorder

Fig. 1

Establishment of homozygous SNTA1-deficient hESCs. A Schematic of the sgRNA designed for the PH1 region in SNTA1 demonstrates that one adenine nucleotide is inserted into SNTA1 before the PAM sequence. B The DNA molecules of H9 and H9SNTA1KO cells were detected by DNA sequencing. The DNA was extracted and amplified by PCR. The result showed one adenine nucleotide was inserted into the SNTA1 before the PAM sequence in the H9SNTA1KO genome. C Utilizing tools from the Synthego website to assess the gene-editing ratio. The upper graph offered the abstract of the indel ratio with the results showing a knockout score of 99%. The relative contribution of the sequence is a nucleotide was inserted before the PAM sequence. The graph below offered the analysis of the sequence using the Synthego tools. The red dotted line is under the PAM sequence in the control sample diagram. The vertical dotted line demonstrated one adenine inserted in the edited sample diagram. The homozygous SNTA1 knockout hESCs were established. D Immunofluorescence staining for pluripotency was performed. Both SSEA4 and NANOG were positive in H9SNTA1KO. SNTA1-knockout did not influence the pluripotency of hESCs

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