Fig. 2

H9SNTA1KO differentiated into cardiomyocytes. A Schematic of hESCs induction into cardiomyocytes using small molecule inhibitors. B Image B1–B3 were hESCs induction into cardiomyocytes using working solutions. Scale bar: 100 μm. Image B4 showed the mass of beating cardiomyocytes on the 10th day of differentiation. Scale bar: 100 μm. C The image of KO exhibited purified by metabolic selection using a glucose-free medium supplemented with lactate. Scale bar: 50 μm. D Immunostaining of TNNT2 (green) and α-actinin (red) in KO. Scale bar: 7.5 μm. E The left graph: Flow cytometry was used to detect a specific cardiac marker, TNNT2. The result demonstrated that the differentiation rate of H9SNTA1KO was similar to the WT without purification. The right graph: Quantification of TNNT2 based on flow cytometry (n = 3). ns; not significant, unpaired two-sided Student’s t test. F The left graph: Flow cytometry was used to detect a specific ventricular muscle marker, MYL2. The results demonstrated that the yield of WT and KO was similarly purified using metabolic selection. The right graph: Quantification of MYL2 of the flow cytometry (n = 3). ns; not significant, unpaired two-sided Student’s t test