Fig. 4

Exhibiting hypertrophic phenotype in KO-cardiomyocytes. A Comparison of NPPA at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes by qRT-PCR. n = 3 independent experiments. *P < 0.05. B Comparison of NPPB at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes by qRT-PCR. n = 3 independent experiments. **P < 0.01. C Comparison of MYH6 at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes by qRT-PCR. n = 3 independent experiments. ***P < 0.001. D Comparison of MYH7 at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes by qRT-PCR. n = 3 independent experiments. **P < 0.01. E Comparison of the ratio of MYH7 to MYH6 at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes. n = 3 independent experiments. **P < 0.01. F Immunoblot analysis of MYH7 in WT-cardiomyocytes and KO-cardiomyocytes. G Comparison of MYL2 at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes by qRT-PCR. n = 3 independent experiments. **P < 0.01. H Immunostaining of MYL2 (green) in WT-cardiomyocytes and KO-cardiomyocytes and semi-quantification analysis measured on the 45th day of cardiac differentiation. Scale bar: 10 μm. n = 3 independent experiments. *P < 0.05. I Comparison of TNNT2 at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes by qRT-PCR. n = 3 independent experiments. *P < 0.05. J Immunostaining of TNNT2 (green) in WT-cardiomyocytes and KO-cardiomyocytes and semi-quantification analysis measured on the 45th day of cardiac differentiation. Scale bar: 5 μm. n = 3 independent experiments. **P < 0.01. K Comparison of α-actinin at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes by qRT-PCR. n = 3 independent experiments. *P < 0.05. L Immunostaining of α-actinin (red) in WT-cardiomyocytes and KO-cardiomyocytes and semi-quantification analysis measured on the 45th day of cardiac differentiation. Scale bar: 5 μm. n = 3 independent experiments. *P < 0.05. M Immunoblot analysis of MYL2 in WT-cardiomyocytes and KO-cardiomyocytes. N The diameter size of WT-cardiomyocytes and KO-cardiomyocytes were evaluated using flow cytometry on the 66th day of cardiac differentiation