Fig. 7

Impairment of calcium handling in KO-cardiomyocytes. A Heatmap showed the discrepancies in the expression of genes involved in calcium handling in WT-cardiomyocytes and KO-cardiomyocytes. B Comparison of SERCA2a at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes by qRT-PCR. n = 3 independent experiments. *P < 0.05. C Immunoblot analysis of SERCA2a in WT-cardiomyocytes and KO-cardiomyocytes. D Comparison of BIN1 at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes by qRT-PCR. n = 3 independent experiments. *P < 0.05. E Immunoblot analysis of BIN1 in WT-cardiomyocytes and KO-cardiomyocytes. F Comparison of CASQ2 at transcriptional level between WT-cardiomyocytes and KO-cardiomyocytes by qRT-PCR. n = 3 independent experiments. *P < 0.05. G Immunoblot analysis of CASQ2 in WT-cardiomyocytes and KO-cardiomyocytes