Fig. 2

Enforced expression of OSMR in progenitor cells suppressed osteogenesis and promoted adipogenesis. Overexpression of OSMR in ST2 was verified using qRT-PCR (A) and Western blotting (B). The effect of OSMR overexpression on the viability of ST2 cells was detected by CCK-8 assay (C). ALP staining (D) and alizarin red staining (E) of differentiated osteoblasts were done. The mRNA (F) and protein (G) levels of osteogenic factors were examined. Lipid droplet formation in differentiated adipocytes was detected by staining with oil-red O (H). Oil-red O dye was extracted and absorbance was measured by spectrophotometry at 520 nm (I). The mRNA (J) and protein (K) levels of adipogenic factors were examined. Scale bar in (H): 50 μm. Values are mean ± SD. A, B, F, G, I–K, n = 3. C, n = 6. *p < 0.05 vs. Vector