Fig. 7

OSMR silencing attenuated osteoclast differentiation in ovariectomized mice. Bone marrow cells were isolated from the transplanted mice, cultured, and induced to allow osteoclast differentiation. TRAP staining was performed (A), osteoclasts were counted (B), and the mRNA and/or protein expression levels of osteoclastogenic factors were examined (C, D). Scale bar in (A): 200 μm. TRAP staining was performed on the transplanted tibia sections and representative images are shown (E). Scale bar: 50 μm. The number (F) and surface (G) of the osteoclasts on the trabeculae were quantified. Schematic diagram depicting the mechanism for OSMR in regulating osteoblast and osteoclast differentiation is shown (H). Values are mean ± SD. B, n = 4; C, D, n = 3; E, F, n = 5. *p < 0.05 vs. Sham/Ctrl LV. ^#p < 0.05 vs. OVX/Ctrl LV