Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: KAP1 phosphorylation promotes the survival of neural stem cells after ischemia/reperfusion by maintaining the stability of PCNA

Fig. 1

The expression of KAP1 and p-KAP1 in NSCs after cerebral I/R. A The expression and location of KAP1 in the subgranular zone (SGZ) and Subventricular zone (SVZ) were examined by immunofluorescence staining. KAP1 was dyed red, Nestin was green and the nuclear was stained blue with DAPI. 100× , scale bar = 200 μm; 200 × , scale bar = 50 μm. B The phosphorylation and protein expression of KAP1 in SVZ of rat subjected to I/R were analyzed by immunofluorescence staining. 200 × , scale bar = 200 μm. C Western blot analysis was also utilized to detect the phosphorylation and protein expression levels of KAP1 in C17.2-NSCs after OGD/R. D Bands were scanned, and the intensities were represented as folds of normoxia group, ***P < 0.001 versus normoxia group (N means normoxia, O/R means OGD/R). Data were expressed as mean ± SEM (n = 5). E Immunofluorescence staining was also performed to detect phosphorylation and protein expression. 200 × , scale bar = 100 μm

Back to article page