Fig. 2

Effects of p-KAP1 on the proliferation of NSCs subjected to cerebral I/R. A BrdU immunofluorescence staining was performed to evaluate the C17.2-NSCs proliferation. 200 × , scale bar = 100 μm. B CCK-8 assay was also used to detect the proliferation of C17.2-NSCs. Data were presented as mean ± SEM (n = 3). ^**P < 0.01 versus WT and O/R group; ^&P < 0.05 versus SD and O/R group. C, D Western blot analysis was used to examine the expression of PCNA. Band intensities were represented as fold-values relative to the levels in normoxia treatment. Data were presented as mean ± SEM (n = 4), ^*P < 0.05, ^**P < 0.01 versus WT and O/R group; ^&P < 0.05 versus SD and O/R group. E Ki-67 and Nestin colocalization immunofluorescence staining was performed to evaluate the neurogenesis of endogenous NSCs. 200× , scale bar = 100 μm