Fig. 4

ADSC-sEVs treatment inhibited the disruption of BSCB following SCIRI. a Representative images of EB extravasation in the spinal cord sections. b Quantification of the intensity of Evans blue. The fluorescence intensity of EB is represented as the average integrated optical density (IOD). c Quantification of the amount of Evans blue in the spinal cord tissues. d Representative images showing double immunofluorescence of ZO-1 and CD31. e Expression of tight junction proteins in each experimental group was determined by Western blotting assay. f Relative expression levels of tight junction proteins normalized to GAPDH. *p < 0.05 compared with the Sham group, ^#p < 0.05 compared with the SCIRI group