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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Endoplasmic reticulum stress modulates the fate of lung resident mesenchymal stem cell to myofibroblast via C/EBP homologous protein during pulmonary fibrosis

Fig. 2

LR-MSC in bleomycin-induced pulmonary fibrosis (BLM) mirrors the behavior of which in IPF. A Immunofluorescence of BLM and normal lungs show the colocalization of mouse LR-MSC marker Sca1 and myofibroblast marker α-SMA. Arrows indicate positive staining of α-SMA in LR-MSC. Arrowheads indicate the parabronchial distribution of LR-MSC during homeostasis. B Immunofluorescence of BLM and normal lungs show the expression of CHOP in LR-MSC. C Gating strategy for analyzing the expression of EpCam in Sca1+CD45−CD31− cells by FACS. D Isolated LR-MSC morphology was revealed by conventional light microscopy after 3 days of culture. E Schematic of the workflow used to isolate LR-MSC during homeostasis (Saline) and fibrosis (Bleomycin) for subsequent experiments. F Counts of isolated LR-MSC in each normal or BLM C57BL/6 mouse. G Western blot shows the expression of ER stress and fibrosis-associated proteins in isolated LR-MSC

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Stem Cell Research & Therapy

ISSN: 1757-6512