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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Endoplasmic reticulum stress modulates the fate of lung resident mesenchymal stem cell to myofibroblast via C/EBP homologous protein during pulmonary fibrosis

Fig. 5

CHOP overexpression facilitates the TGFβ1-induced myofibroblast differentiation of LR-MSC by influencing the TGFβ/SMAD signaling pathway. A Heat map for the mRNA level of TGFβ/SMAD signaling-associated genes by qPCR. Data are shown relative to untreated LR-MSC. B The activation of TGFβ1/SMAD signaling was evaluated by the transfection LR-MSC with SMAD reporter plasmid using luciferase assay. C The level of Smad2/3 or phosphorylated Smad2/3 was evaluated by Western blot. D Co-IP analysis for the binding of C/EBPβ with CBP and CHOP, and binding of CBP with Smad2/3. E The schematic diagram illustrates the molecular mechanism by which CHOP promotes the transcriptional activity of the SMAD complex by affecting the competitive binding of C/EBPβ and Smads to CBP. F The effects of CHOP interference on the Wnt/β-catenin signaling pathway were evaluated by Western blot

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Stem Cell Research & Therapy

ISSN: 1757-6512