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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Endoplasmic reticulum stress modulates the fate of lung resident mesenchymal stem cell to myofibroblast via C/EBP homologous protein during pulmonary fibrosis

Fig. 6

CHOP knockdown inhibits the myofibroblast differentiation of LR-MSC in vivo and moderates bleomycin-induced pulmonary fibrosis. A Schematic of the workflow used to treat mice. B Kaplan–Meier survival plot for mice treated with bleomycin (n = 6 per group). C Representative image for HE and Masson’s staining for saline, bleomycin, and LR-MSC treated mice after 21 days. D Ashcroft’s score (fibrosis) and E collagen volume fraction in different groups were shown. F mRNA level for fibrosis-associated genes was quantified by qPCR. G Hydroxyproline contents in lung tissue were quantified by ELISA. H Fluorescence intensity for LR-MSC-expressed GFP in different groups was tested by IVIS lumina II. I The mRNA level for fibrosis-associated genes in sorted GFP+ LR-MSC were tested by qPCR. J Immunofluorescence for the consecutive slides of LR-MSC and LR-MSC-shCHOP-treated BLM mouse lung showed the expression of Grp78, α-SMA, and Collagen I in GFP-positive LR-MSC. Arrows and arrowheads in different panels indicate the same LR-MSC, respectively. K Comparison for the counts of α-SMA-positive and L Grp78-positive LR-MSC in each filed

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Stem Cell Research & Therapy

ISSN: 1757-6512