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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Hypoxia drives hematopoiesis with the enhancement of T lineage through eliciting arterial specification of hematopoietic endothelial progenitors from hESC

Fig. 5

Hematopoietic potential of the hESC-derived CD45+ cells in vitro. A CB CD34+ cells and CD45+ cells derived from hESCs at day 15 after differentiation from hESCs were collected for MethoCult colony assays. B The colony-forming potential of CD45+ cells derived from hESCs was compared with those from CB CD34+ cell populations, and the number of CFU-GEMM, CFU-E and CFU-G/M was scored 2 weeks after the colony-forming. C All colonies were isolated and surface phenotypes of the cells from these colonies was assessed by flow cytometry. D Colonies were isolated for analyzing the expression of megakaryocytic gene (LMO2, SCL and GATA2), erythroid genes (huHbB, huHbG and huHbE), granulocyte (GYPA) and HPCs genes (GATA1) by RT-qPCR (n = 3, mean ± SEM). E T cell potential of HPCs generated from hESCs at day 15 after differentiation from hESCs following 4 weeks of inductive culture for production of T cells. Data represent the mean ± SEM. *p < 0.05, **p < 0.01, and ns (no significance)

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Stem Cell Research & Therapy

ISSN: 1757-6512