Skip to main content
Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment

Fig. 3

Ad5-Ki67/IL-15 effectively infected and killed GA-MSCs in vitro. A The GA-MSCs treated with the virus (MOI = 40) for 48 h, the representative images were acquired using fluorescence microscopy (× 100, scale bars = 1000 µm). B and C The cell cycle distribution of GA-MSCs treated with Ad5-Ki67/IL-15. D and E CCK-8 assay was performed to evaluate cell growth of GA-MSCs that treated with Ad5-Ki67/IL15 (MOI = 40) for 24, 48, 72 h. Ad5-Ki67/IL15 could inhibit the proliferation of GA-MSCs compared to control. F-G The proliferation of GA-MSCs in response to the conditioned medium from the virus-treated U251 or BT-01 cells (U251/BT-01-Ad5-Ki67/IL15-CM, MOI = 40) at different time points was determined by CCK8. The conditioned medium of glioma cell-treated with the virus significantly suppressed the cell growth compared with the untreated. All data are presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Back to article page