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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Tongxinluo-pretreated mesenchymal stem cells facilitate cardiac repair via exosomal transfer of miR-146a-5p targeting IRAK1/NF-κB p65 pathway

Fig. 7

miR-146a-5p protected H9C2 cells from hypoxia injury by downregulating IRAK1 and inhibiting the nuclear translocation of the NF-κB p65 subunit. A The miR-146a-5p level of H9C2 cells was significantly reduced under H/SD conditions (n = 5). miR-146a-5p mimics significantly increased (B) or miR-146a-5p inhibitors significantly decreased (C) miR-146a-5p levels in H9C2 cells (n = 3). Representative images of the flow cytometry assay (D) and quantification (E) of apoptotic H9C2 cells transfected with miR-146a-5p mimics, inhibitors or the corresponding negative control (n = 5). F Luciferase activity assay of miR-146a-5p mimics-treated HEK293T cells, which overexpressed either IRAK1-wild-type or IRAK1-mutant. Under H/SD conditions, both the mRNA (G, n = 4) and protein levels of IRAK1 (H-I, n = 4) were obviously reduced. J The mRNA level of Irak1 in H9C2 cells transfected with miR-146a-5p mimics, inhibitors or NC (n = 4). Representative western blotting images (K) and quantification (L) of the relative level of IRAK1 (n = 4). Western blotting images (M or O) and quantification (N or P) of the relative level of IRAK1 in MSCsTXL treatment in vivo or in vitro. Representative images (Q) and quantification (R) of the relative level of NF-κB p65 (n = 3). Representative images of flow cytometry (S) and quantification (T) of apoptotic H9C2 cells transfected with IRAK1 siRNA or its NC (n = 4). Representative western blotting images (U) and quantification (V) of nuclear NF-κB p65 levels in H9C2 cells transfected with IRAK1 siRNA or its NC (n = 3). All data are presented as the mean ± SD. Statistical analysis was performed with one-way ANOVA or student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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