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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: A novel hypoxic lncRNA, HRL-SC, promotes the proliferation and migration of human dental pulp stem cells through the PI3K/AKT signaling pathway

Fig. 1

Identification of hDPSCs and promotion of hypoxia on hDPSCs. a, b Primary cultured and passage hDPSCs. Alizarin Red and ALP activity staining was performed and mineral nodes (c) and purple-colored precipitate (d) were detected in the induced group but not appeared in the non-induced group (e, f). g Surface markers of hDPSCs were detected by flow cytometric analysis, hDPSCs were positive for CD44, CD29, CD90 and negative for CD45, CD34. h EdU assay of cell proliferation. EdU-positive cells were in red and cell nuclei were dyed in blue. i EdU-positive cell ratio in normoxia group vs. hypoxia group. j Migration capability was characterized by scratch wound healing assay. k Scratch wound healing area in normoxia group vs. hypoxia group. ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512