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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Dichotomous role of Shp2 for naïve and primed pluripotency maintenance in embryonic stem cells

Fig. 2

Role of Shp2 in naïve pluripotency. A Graphical illustration of isogenic pair of naïve (OG2) and primed (POG2) ESCs (left), brightfield and fluorescent microscopic images of OG2 and POG2 ESCs (scale bars, 500 μm) (right panel). B Representative light microscopic images of WT and KD ESCs (scale bars, 200 μm) (left panel) and fluorescent microscopic images of WT and KD ESCs (scale bars, 500 μm) (right panel). C Fold mRNA expression of typical naïve pluripotency marker genes in WT and KD ESCs (**p < 0.01 and ***p < 0.001, n = 3). D Fluorescent microscopic images of WT and KD ESCs at indicated time under LIF + 2i and LIF only culture condition (scale bars, 500 μm). E Immunoblotting for pStat3, Stat3 and Erk2 in WT and KD ESCs at indicated time after LIF stimulation (1000 U/ml), Erk2 was used as a loading control. LIF starvation for 1 h was performed before the LIF stimulation. F Immunoblotting of OG2 mESCs with control (siNC) or Ptpn11 siRNA (siPtpn11) at indicated time after LIF stimulation (1000 U/ml), α-tubulin was used as a loading control. LIF starvation for 1 h was performed before the LIF stimulation. G The normalized enrichment score by gene set enrichment analysis (GSEA) of WT and KD ESCs for Hallmark_IL6_JAK_STAT3 signaling (left panel), KEGG_JAK_STAT3 signaling (middle panel), and ABBUD_LIF signaling up (right panel). H DEGs of WT LIF + 2i vs WT (-)2i were assigned as ‘gene sets for 2i’. The number of Up-DEGs and Down-DEGs within the ‘gene sets for 2i’ group are presented. I Light microscopic images of WT and KD 48 h after indicated culture condition (scale bars, 200 µm)

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