Table 1 Sequences of primers for quantitative real-time PCR
From: Dichotomous role of Shp2 for naïve and primed pluripotency maintenance in embryonic stem cells
Primer pairs | Primer sequences (5’-3’) | |
|---|---|---|
Ptpn11 | Forward | AGTCCAAAGTGACCCACGTC |
Reverse | CCATCATGCAGAACGACCCT | |
Dppa3 | Forward | CGTACCTGTGGAGAACAAGAGTG |
Reverse | CA TTCTCAGAGGGA TCCCA TCTTTG | |
Rex1 | Forward | CTTCGAAAGCTTGGAGGAAGTGGAG |
Reverse | GGACACTCCAGCATCGATAAGACAC | |
Nr0b1 | Forward | ACAGAGCAGCCACAGA TGGTGTC |
Reverse | GATGTGCTCAGTAAGGATCTGCTG | |
Klf4 | Forward | GAACAGCCACCCACACTTGTGAC |
Reverse | CTGTCACACTTCTGGCACTGAAAG | |
Esrrb | Forward | GATTCTCATCTTGGGCATCGTGTAC |
Reverse | CTGACTCAGCTCATAGTCCTGCAG | |
Klf2 | Forward | CACACATACTTGCAGCTACACCAAC |
Reverse | CAAGTGGCACTGAAAGGGTCTGTG | |
Fgf5 | Forward | CATCGGTTTCCATCTGCAGATCTAC |
Reverse | GTTCTGTGGATCGCGGACGCATAG | |
Cer1 | Forward | GTGGAAAGCGA TCA TGTCTCA TCG |
Reverse | GCAAAGGTTGTTCTGGACAACGAC | |
Pou5f1 | Forward | GAGAAAGCGAACTAGCATTGAGAAC |
Reverse | TGTAGCCTCATACTCTTCTCGTTG | |
Sox2 | Forward | ATGGGCTCTGTGGTCAAGTC |
Reverse | CCCTCCCAATTCCCTTGTAT | |
Nanog | Forward | GTGCACTCAAGGACAGGTTTCAG |
Reverse | CTGCAATGGATGCTGGGATACTC | |
c-Myc | Forward | ACCACCAGCAGCGACTCTGA |
Reverse | TGCCTCTTCTCCACAGACACC | |