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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Mesenchymal stem cells-derived small extracellular vesicles alleviate diabetic retinopathy by delivering NEDD4

Fig. 6

MSC-sEV-delivered NEDD4 promoted PTEN ubiquitination and degradation. A Western blot analysis for the expression of NEDD4 in RPE cells treated with MG132. B Mass spectrometry analysis results of MSC-sEV protein content. C Western blot analysis for the expression of NEDD4 in MSC-sEV and HFL1-sEV. D Representative immunohistochemistry staining images of NEDD4 in retinal tissues after treatment. Scale bars, 100 μm. E Western blot analysis for the expression of NEDD4 in retinal tissues after treatment. F Western blot analysis for the expression of NEDD4 in RPE cells after treatment. G Representative immunofluorescence staining images of NEDD4 in RPE cells after treatment. Scale bars, 25 μm. H The ubiquitination of PTEN protein after MSC-sEV treatment and the binding of NEDD4 and PTEN were detected by co-IP assay. All data are presented as means ± SEM. ns, not significant, ***P < 0.001

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