Skip to main content
Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: IFN-γ enhances the efficacy of mesenchymal stromal cell-derived exosomes via miR-21 in myocardial infarction rats

Fig. 5

miR-21 was a key component in IFN-γ-Exo-induced cardioprotection. Quantitative real-time PCR (qRT-PCR) analysis of miR-21 level in control and IFN-γ-primed MSCs (A) and Exos (B) (n = 3). C Quantitative real-time PCR (qRT-PCR) analysis of miR-21 level among groups at 3 days post-MI. (n = 6) (D) Heat map based on mRNAs sequence values (red represents high expression and the green represents low expression) between control and IFN-γ-primed MSCs. E Bioinformatic analysis network of the transcription factors interacting with the promoter of miR‐21. The yellow circle represents hsa‐miR‐21, and the blue and red circles represent transcription factors. F STAT1, STAT2, and FOXC1 were present in both up-regulated transcription factors and bioinformatics prediction analysis results. G qRT-PCR analysis of STAT1 level in MSCs treated with control and IFN-γ (n = 3). H qRT-PCR analysis of miR-21-5p level in MSCs treated with control, IFN-γ, IFN-γ + si-STAT1 (n = 3). I Luciferase reporter assay was used to detect the miR-21 promoter-reporter activity in 293T cells transfected with vector or STAT1 (n = 3). Data were presented as mean ± SEM. Statistical analysis was performed with one-way ANOVA followed by Bonferroni’s correction. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

Back to article page