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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Exosomal lncRNA TUG1 derived from human urine-derived stem cells attenuates renal ischemia/reperfusion injury by interacting with SRSF1 to regulate ASCL4-mediated ferroptosis

Fig. 5

LncRNA TUG1 carried by USC-Exo suppressed H/R-induced ferroptosis in HK-2 cells. A The expression of TUG1 in isolated USC-Exo was measured by qPCR. B The uptake of USC-Exo by HK-2 cells was checked by PKH67 immunofluorescence dyes. Scale bar: 100 µm. C The effect of USC-Exo on the expression of TUG1 in HK-2 cells was detected by qPCR. D The expression of TUG1 in USCs (untreated, transfection with sh-NC and sh-TUG1): USC-Exo, sh-NC-treated USC-Exo, and TUG1-silenced USC-Exo, was checked by qPCR. NC: negative control. E Cell viability in HK-2 cells after treated with H/R and sh-NC-treated USC-Exo and TUG1-silenced USC-Exo was measured by CCK-8 kits. F Cell death in HK-2 cells was checked by TUNEL assay. TUNEL-positive cell quantitative bar diagrams are shown. Scale bar: 100 µm. G The levels of MDA and GSH in HK-2 cells were assessed by using commercial kits. H The levels of Fe2+ in HK-2 cells were assessed by using commercial kits. I The levels of ROS in HK-2 cells were detected using DCFH-DA probe. J The protein levels of ACSL4, GPX4, FTH1, and COX2 in HK-2 cells were detected by western blot and shown as blot image and quantitative bar diagram. N = 3 samples per groups. *p < 0.05, **p < 0.01, ***p < 0.001. The above data were all measurement data and expressed as means ± SD. All experiment was performed three times

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