Fig. 6

LncRNA TUG1 regulates the stability of mRNA by interacting with SRSF1. A, B The localization of TUG1 in HK-2 cells was determined by subcellular fractionation assay and RNA FISH assay. Scale bar: 10 µm. C The schematic illustration of RNA pull-down assay. Interaction between lncRNA TUG1 and SRSF1 was confirmed by D RNA pull-down and E RIP assay. F Schematic illustration of SRSF1 full-length protein and truncations; their expression in E. coli was shown in the SDS-PAGE gel below. G The binding of TUG1 to SRSF1 full-length protein and truncated proteins was assessed by RIP. H The effect of USC-Exo on the expression of ACSL4 was checked by qPCR and western blot. I The effect of TUG1 and SRSF1 on the ACSL4 expression was assessed by qPCR and western blot. J RIP to determine the interaction between ACSL4 mRNA and SRSF1 protein using anti-SRSF1 or IgG (negative control) in HK2 cells in the presence or absence of TUG1 overexpression. K The effect of TUG1 and SRSF1 on the stability of ACSL4 mRNA was measured by qPCR. N = 3 samples per groups. *p < 0.05, **p < 0.01, ***p < 0.001. The above data were all measurement data and expressed as means ± SD. All experiment was performed three times. Sen., sense; As., antisense