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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Anti-fibrotic effect of a selective estrogen receptor modulator in systemic sclerosis

Fig. 1

Characterization of induced pluripotent stem cells (iPSCs) from patients with systemic sclerosis (SSc) and healthy controls. a Morphology of iPSCs, as determined by Leica microscopy. Scale bars, 200 μm. b Alkaline phosphatase staining of iPSCs. Scale bars, 200 μm. c Immunocytochemical analysis of iPSC expression of the pluripotency-marker proteins Oct4, SSEA4, Sox2, TRA-1-60, Klf4, and TRA-1-81. Scale bars, 200 μm. d H&E staining of the teratomas generated from the iPSCs. e RT-PCR analysis of iPSC expression of the pluripotency-marker genes OCT4, SOX2, NANOG, and LIN28. All graphs show the mean and standard error of the mean of five replicates of each of the iPSC lines (*p < 0.05, **p < 0.01, ***p < 0.001, as determined by Student’s t test)

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