Biodistribution of allogenic umbilical cord-derived mesenchymal stromal cells after fetal repair of myelomeningocele in an ovine model

Table 1 Q-PCR experiment among DNA from GFP-tagged UC-MSCs diluted in DNA from non GFP-tagged UC-MSCs

Dilution		Concentration of DNA GFP + in DNA GFP-(ng/µL)	GFP applied biosystems™ TaqMan™ gene expression assay		RHOB applied biosystems™ TaqMan™ gene expression assay
Dilution		Concentration of DNA GFP + in DNA GFP-(ng/µL)	Mean CT ± SD	CV (%)	Mean CT ± SD	CV (%)
1	1	4	26.5 ± 0.3	1.3	27.6 ± 0.07	0.2
2	1/2	2	27.9 ± 0.1	0.4	27.5 ± 0.06	0.2
3	1/5	0.8	30.8 ± 0.3	1.0	27.4 ± 0.08	0.3
4	1/10	0.4	31.3 ± 1.4	4.4	27.2 ± 0.05	0.2
5	1/100	0.04	33.9 ± 0.6	1.7	27.1 ± 0.09	0.3
6	1/1000	0.004	35.9 ± 0.8	2.2	27.2 ± 0.06	0.2
7	1/10000	0.0004	36.2 ± 0.5	1.4	27.1 ± 0.05	0.2
8	1/100000	0.00004	36.5 ± 0.9	2.5	26.9 ± 0.01	0.03
9	1/1000000	0.000004	ND	ND	27.0 + /0.04	0.1

CT, cycle threshold; SD, standard deviation; ND, not detected; GFP, green fluorescent protein, RHOB, Ras homolog gene family

ISSN: 1757-6512